Supplemental Protein Influences on Carbohydrate Degradation and Bacterial 16S Ribosomal Ribonucleic Acid
نویسندگان
چکیده
منابع مشابه
Agglutination of bacterial spheroplasts: agglutination-dependent degradation of Escherichia coli ribosomal ribonucleic acid.
When Escherichia coli spheroplasts made by ethylenediaminetetraacetic acid and lysozyme were agglutinated by concanavalin A (con A), the degradation of ribosomal ribonucleic acid (rRNA) was found to occur proportionally to the degree of the agglutination, which was determined by microscopic examination or by a newly devised assay based on the slower settling of aggregates. Methyl-alpha-d-glucos...
متن کاملLocation of ribosomal protein binding sites on 16S ribosomal RNA.
The distribution of ribosomal protein binding sites on the 16S ribosomal RNA molecule has been analyzed by limited ribonuclease hydrolysis of RNA-protein complexes, as well as by the interaction of individual proteins with RNA fragments purified from partial enzymatic digests. Of the six 30S subunit proteins known to interact directly with 16S RNA, proteins S4, S8, S15, S20, and, probably, S13 ...
متن کاملMolecular integrity of plant ribosomal ribonucleic Acid.
Thermal denaturation of plant ribosomal RNA followed by gel fractionation shows that although a large percentage of molecules contain breaks in the polynucleotide chain, 25S and 18S RNAs do exist as unique molecular species. Values for the rate constant of hydrolysis under routine denaturing conditions are of the order of 10(-7) to 10(-8) sec(- 1) and these are shown not to be a result of ribon...
متن کاملConformational Changes in Ribosomal Ribonucleic Acid
Both rat liver and Escherichia coli rRNA in 0.1M-sodium chloride were titrated with acid or alkali over the range pH 3-7 at approx. 0°C. rRNA did not bind acid reversibly and hysteresis was observed, i.e. the plot of acid bound to rRNA against pH had the form of a loop showing that the amount of acid bound at a particular pH depended on the direction of the titration. Although the boundary curv...
متن کاملRibonucleic acid metabolism in lymphocytes stimulated by phytohemagglutinin. II. Rapidly synthesized ribonucleic acid and te production of ribosomal ribonucleic acid.
Human peripheral lymphocytes are stimulated to change, in vitro, from a resting state to one of progressive enlargement, culminating in mitosis, by treatment with Phaseolus vulgaris phytohemagglutinin. The sedimentation characteristics and fate of rapidly synthesized RNA have been studied in this system after 20 hours of stimulation with phytohemagglutinin, a time when RNA synthesis has been ac...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1993
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(93)77687-0